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Forecast method for used number of parts and components based on complex network
LIU Fu-yun, QI Guo-ning, YANG Qing-hai
《机械工程前沿(英文)》 2006年 第1卷 第4期 页码 479-484 doi: 10.1007/s11465-006-0064-5
关键词: complex network industrial component analytic expression forecast
Practical evaluation for water utilities in China by using analytic hierarchy process
Hong CHEN,Feng XIAO,Zhe BI,Ping XIAO,Dongsheng WANG,Ming YANG
《环境科学与工程前沿(英文)》 2015年 第9卷 第1期 页码 131-137 doi: 10.1007/s11783-014-0718-1
关键词: analytic hierarchy process (AHP) risk management water quality failure water quantity failure
Shuangxi FENG, Huayang LEI, Yongfeng WAN, Haiyan JIN, Jun HAN
《结构与土木工程前沿(英文)》 2021年 第15卷 第2期 页码 461-477 doi: 10.1007/s11709-021-0705-0
关键词: deep foundation pit excavation adjacent bridge foundation influencing factors analytic hierarchy process finite element
Application of fuzzy analytic hierarchy process model on determination of optimized pile-type
Lei MA, Shuilong SHEN, Jinhui ZHANG, Yang HUANG, Feng SHI
《结构与土木工程前沿(英文)》 2010年 第4卷 第2期 页码 252-257 doi: 10.1007/s11709-010-0017-2
关键词: pile type fuzzy synthetic evaluation analytic hierarchy process (AHP) optimal selection
Blockage of receptor-interacting protein 2 expression by small interfering RNA in murine macrophages
LIU Hongchun, CAO Zhongwei, JIN Jianjun, WANG Jiyao
《医学前沿(英文)》 2008年 第2卷 第2期 页码 166-170 doi: 10.1007/s11684-008-0030-1
MSI/LOH and extron expression of the FHIT gene in gastric carcinoma
XIAO Yuping, MAO Lili, HAN Chengbo, LI Jinyi, XU Lei, XIN Yan
《医学前沿(英文)》 2007年 第1卷 第1期 页码 99-103 doi: 10.1007/s11684-007-0019-1
Gene expression disparity in giant cell tumor of bone
Xiaohua PAN, Shuhua YANG, Deming XIAO, Yong DAI, Lili REN
《医学前沿(英文)》 2009年 第3卷 第1期 页码 49-56 doi: 10.1007/s11684-009-0012-y
《医学前沿(英文)》 2022年 第16卷 第4期 页码 627-636 doi: 10.1007/s11684-020-0815-4
关键词: RUNX1 gene mutation acute myeloid leukemia transcriptional repression DNA methylation
Immunohistochemical measurement and expression of Mcl-1 in infertile testes
null
《医学前沿(英文)》 2015年 第9卷 第3期 页码 361-367 doi: 10.1007/s11684-015-0395-x
The diagnosis of azoospermia represents a major challenge to andrologists as this condition may occur despite normal spermatogenesis and genital tracts. Myeloid cell leukemia-1 (Mcl-1) is a member of the Bcl-2 family of proteins involved in regulation of apoptosis in various cell types. This study aimed to investigate the immunohistochemical expression of Mcl-1 in testicular biopsies of subjects with azoospermia. Eighty-six cases with azoospermia were obtained from 509 infertile patients admitted to the Andrology Unit of the Zagazig University Hospitals from January 2010 to December 2011. Biopsies were diagnosed and classified using H&E-stained slide sections. The specimens were subjected to immunohistochemical staining for Mcl-1 and examined through light microscopy. Forty-five cases of maturation arrest (25 at spermatids and 20 at the spermatocytes), 31 cases of hypospermatogenesis (20 moderate and 11 severe), 5 cases of Sertoli?cell-only syndrome, 2 cases of basement membrane hyalinization, and 1 case of tubular and peritubular sclerosis were observed. Normal spermatogenesis was detected in 2 cases. A strong positive immunoreaction in Leydig cells was observed among all investigated specimens. A moderate reaction was detected in spermatocytes and spermatozoa in cases of normal spermatogenesis and hypospermatogenesis, but a negative reaction was detected in cases of maturation arrest and germ cell aplasia. Apoptosis was found to be associated with decreased rate of spermatogenesis. High apoptosis rates may result in azoospermia, which can occur despite normal spermatogenesis and absence of duct obstruction.
关键词: spermatogenesis testis Mcl-1 expression immunohistochemical study infertility
Shen LIU, Shengzhe SHANG, Xuezhen YANG, Huihua ZHANG, Dan LU, Ning LI
《农业科学与工程前沿(英文)》 2018年 第5卷 第3期 页码 382-389 doi: 10.15302/J-FASE-2018211
The mammary gland provides a novel method for producing recombinant proteins in milk of transgenic animals. A key component in the technology is the construction of an efficient milk expression vector. Here, we established a simple method to construct a milk expression vector, by a combination of homologous recombination and digestion-ligation. Our methodology is expected to have the advantages of both plasmid and bacterial artificial chromosome (BAC) vectors. The BAC of mouse whey acidic protein gene (mWAP) was modified twice by homologous recombination to produce a universal expression vector, and the human lysozyme gene (hLZ) was then inserted into the vector by a digestion-ligation method. The final vector containing the 8.5 kb mWAP 5′ promoter, 4.8 kb hLZ genomic DNA, and 8.0 kb mWAP 3′ genomic DNA was microinjected into pronuclei of fertilized mouse embryos, to successfully generate two transgenic mouse lines that expressed recombinant human lysozyme (rhLZ) in milk. The highest expression level of rhLZ was 0.45 g·L−1, and rhLZ exhibited the same antibacterial activity as native hLZ. Our results have provided a simple approach to construct a universal milk expression vector, and demonstrated that the resulting vector regulates the expression of hLZ in milk.
关键词: BAC recombinant methods gene expression human lysozyme transgenic mice milk expression vector
JIANG Yuan, TIAN Xuehong, YUAN Jie, JIN Yuemei, TAN Yusong
《医学前沿(英文)》 2007年 第1卷 第4期 页码 398-400 doi: 10.1007/s11684-007-0077-4
关键词: anticarcinogenic microvessel density malignant potency muscle uterine leiomyosarcoma
Heterologous expression of signal protein 14-3-3 in and the subsequent immune response in mice
ZHENG Meijuan, SHEN Jilong, LUO Qingli, XU Yuanhong
《医学前沿(英文)》 2008年 第2卷 第1期 页码 95-99 doi: 10.1007/s11684-008-0017-y
关键词: development challenging rSj14-3-3 resistance cultural supernatant
张进之,赵厚信,王喆,王保罗
《中国工程科学》 2008年 第10卷 第4期 页码 73-77
连轧张力公式是连轧理论和实践的核心问题,该问题国内外都有大量的研究,20世纪60年代笔者推导了连轧张力微分方程、多机架动态张力公式、稳态张力公式,理论上已作过深入、广泛的分析讨论。日本在20世纪70年代对连轧张力公式进行了理论上的推导和实验验证,特别是浅川基男等人的连轧实验结果更为重要。为此引用了浅川基男的实验数据和宝钢2 050 mm热连轧机上的实测数据,验证了连轧张力公式。
《医学前沿(英文)》 2022年 第16卷 第5期 页码 723-735 doi: 10.1007/s11684-021-0905-y
关键词: amyotrophic lateral sclerosis coronin-1a autophagy pathogenesis
ZHAN Rong, YU Qinghong, HUANG Haobo
《医学前沿(英文)》 2008年 第2卷 第4期 页码 356-360 doi: 10.1007/s11684-008-0068-0
关键词: VEGF expression expression variation culture supernatant labeling concentration
标题 作者 时间 类型 操作
Forecast method for used number of parts and components based on complex network
LIU Fu-yun, QI Guo-ning, YANG Qing-hai
期刊论文
Practical evaluation for water utilities in China by using analytic hierarchy process
Hong CHEN,Feng XIAO,Zhe BI,Ping XIAO,Dongsheng WANG,Ming YANG
期刊论文
Influencing factors and control measures of excavation on adjacent bridge foundation based on analytic
Shuangxi FENG, Huayang LEI, Yongfeng WAN, Haiyan JIN, Jun HAN
期刊论文
Application of fuzzy analytic hierarchy process model on determination of optimized pile-type
Lei MA, Shuilong SHEN, Jinhui ZHANG, Yang HUANG, Feng SHI
期刊论文
Blockage of receptor-interacting protein 2 expression by small interfering RNA in murine macrophages
LIU Hongchun, CAO Zhongwei, JIN Jianjun, WANG Jiyao
期刊论文
MSI/LOH and extron expression of the FHIT gene in gastric carcinoma
XIAO Yuping, MAO Lili, HAN Chengbo, LI Jinyi, XU Lei, XIN Yan
期刊论文
Gene expression disparity in giant cell tumor of bone
Xiaohua PAN, Shuhua YANG, Deming XIAO, Yong DAI, Lili REN
期刊论文
Distinct gene expression pattern of mutations coordinated by target repression and promoter hypermethylation
期刊论文
Construction of a universal recombinant expression vector that regulates the expression of human lysozyme
Shen LIU, Shengzhe SHANG, Xuezhen YANG, Huihua ZHANG, Dan LU, Ning LI
期刊论文
Relationship of adrenomedullin expression and microvessel density and prognosis in smooth muscle tumor
JIANG Yuan, TIAN Xuehong, YUAN Jie, JIN Yuemei, TAN Yusong
期刊论文
Heterologous expression of signal protein 14-3-3 in and the subsequent immune response in mice
ZHENG Meijuan, SHEN Jilong, LUO Qingli, XU Yuanhong
期刊论文
Increased expression of coronin-1a in amyotrophic lateral sclerosis: a potential diagnostic biomarker
期刊论文